Nanobodies are extremely small antibody fragments that bind to specific molecules. When nanobodies are labeled with dyes, they can be used as probes to enhance super-resolution microscopy. However, conventional blinking dyes often perform poorly when attached to nanobodies, limiting their practical advantages. An international research team led by the University of Göttingen and the University Medical Center Göttingen (UMG) has now shown that self-blinking dyes—which can switch between bright and dark states on their own—bypass this limitation.